Review



human normal hepatocytes thle 2  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 98
      Buy from Supplier

    Structured Review

    ATCC human normal hepatocytes thle 2
    The selection of liver cancer cell lines and the WST-8 drug susceptibility test validated the validity of the prognostic features gene. ( A – D ) The mRNA expressions of the four molecules were detected <t>in</t> <t>THLE-2</t> and five liver cancer cell lines using qRT-PCR. ( E and F ) IC50 of lobaplatin for Hep 3B detected in normoxic and hypoxic environments using WST-8. ( G and H ) The IC50 of lobaplatin in SNU-387 cells treated with lobaplatin in normoxic and hypoxic environments was determined by WST-8 method. **P<0.01, ***P<0.001.
    Human Normal Hepatocytes Thle 2, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 641 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+normal+hepatocytes+thle+2/THLE-2/pmc13003803-84-0-18
    Average 98 stars, based on 641 article reviews
    human normal hepatocytes thle 2 - by Bioz Stars, 2026-09
    98/100 stars

    Images

    1) Product Images from "Construction of a Prognostic Model Based on Insulin Resistance-Related Genes to Predict TACE Response and Identification of PD-98059 as a Potential Therapeutic Agent"

    Article Title: Construction of a Prognostic Model Based on Insulin Resistance-Related Genes to Predict TACE Response and Identification of PD-98059 as a Potential Therapeutic Agent

    Journal: Journal of Hepatocellular Carcinoma

    doi: 10.2147/JHC.S553710

    The selection of liver cancer cell lines and the WST-8 drug susceptibility test validated the validity of the prognostic features gene. ( A – D ) The mRNA expressions of the four molecules were detected in THLE-2 and five liver cancer cell lines using qRT-PCR. ( E and F ) IC50 of lobaplatin for Hep 3B detected in normoxic and hypoxic environments using WST-8. ( G and H ) The IC50 of lobaplatin in SNU-387 cells treated with lobaplatin in normoxic and hypoxic environments was determined by WST-8 method. **P<0.01, ***P<0.001.
    Figure Legend Snippet: The selection of liver cancer cell lines and the WST-8 drug susceptibility test validated the validity of the prognostic features gene. ( A – D ) The mRNA expressions of the four molecules were detected in THLE-2 and five liver cancer cell lines using qRT-PCR. ( E and F ) IC50 of lobaplatin for Hep 3B detected in normoxic and hypoxic environments using WST-8. ( G and H ) The IC50 of lobaplatin in SNU-387 cells treated with lobaplatin in normoxic and hypoxic environments was determined by WST-8 method. **P<0.01, ***P<0.001.

    Techniques Used: Selection, Quantitative RT-PCR

    Related Articles

    Selection:

    Article Title: Construction of a Prognostic Model Based on Insulin Resistance-Related Genes to Predict TACE Response and Identification of PD-98059 as a Potential Therapeutic Agent
    Article Snippet: Human normal hepatocytes THLE-2, hepatoma cells Huh-7, HepG2, Hep 3 B, SNU-387, and SK-Hep-1 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Among them, Hep3B is p53-deficient, HepG2 is wild-type p53, and retains many hepatocyte-like metabolic functions., Simultaneously, they were derived from HBV-related HCC.Among them, Hep3B is p53-deficient, HepG2 is wild-type p53, and retains many hepatocyte-like metabolic functions., Simultaneously, they were derived from HBV-related HCC.

    Article Title: Author Correction: Intestinal TM6SF2 protects against metabolic dysfunction-associated steatohepatitis through the gut–liver axis
    Article Snippet: Mouse normal hepatocytes AML-12 cells (RRID: CVCL_0140, CRL-2254), human normal hepatocytes THLE-2 (RRID: CVCL_3803, CRL-2706), human colon epithelial cells Caco2 (RRID: CVCL_0025, HTB-37) and human HEK293T cells (RRID: CVCL_0063, CRL-3216) were purchased from the American Type Culture Collection.. AML-12 cells were cultured in DMEM/F-12 medium (Gibco) supplemented with 10% FBS (Thermo Fisher Scientific), 5 mg ml−1 insulin, 5 μg ml−1 transferrin, 5 ng ml−1 selenium, 40 ng ml−1 dexamethasone and 1% penicillin–streptomycin (Thermo Fisher Scientific).AML-12 cells were cultured in DMEM/F-12 medium (Gibco) supplemented with 10% FBS (Thermo Fisher Scientific), 5 mg ml−1 insulin, 5 μg ml−1 transferrin, 5 ng ml−1 selenium, 40 ng ml−1 dexamethasone and 1% penicillin–streptomycin (Thermo Fisher Scientific).

    Article Title: Intestinal TM6SF2 protects against metabolic dysfunction-associated steatohepatitis through the gut–liver axis
    Article Snippet: Mouse normal hepatocytes AML-12 cells (RRID: CVCL_0140, CRL-2254), human normal hepatocytes THLE-2 (RRID: CVCL_3803, CRL-2706), human colon epithelial cells Caco2 (RRID: CVCL_0025, HTB-37) and human HEK293T cells (RRID: CVCL_0063, CRL-3216) were purchased from the American Type Culture Collection.. AML-12 cells were cultured in DMEM/F-12 medium (Gibco) supplemented with 10% FBS (Thermo Fisher Scientific), 5 mg ml −1 insulin, 5 μg ml −1 transferrin, 5 ng ml −1 selenium, 40 ng ml −1 dexamethasone and 1% penicillin–streptomycin (Thermo Fisher Scientific).AML-12 cells were cultured in DMEM/F-12 medium (Gibco) supplemented with 10% FBS (Thermo Fisher Scientific), 5 mg ml −1 insulin, 5 μg ml −1 transferrin, 5 ng ml −1 selenium, 40 ng ml −1 dexamethasone and 1% penicillin–streptomycin (Thermo Fisher Scientific).

    Article Title: Intestinal TM6SF2 protects against metabolic dysfunction-associated steatohepatitis through the gut-liver axis.
    Article Snippet: Mouse normal hepatocytes AML-12 cells (RRID: CVCL_0140, CRL-2254), human normal hepatocytes THLE-2 (RRID: CVCL_3803, CRL-2706), human colon epithelial cells Caco2 (RRID: CVCL_0025, HTB-37) and human HEK293T cells (RRID: CVCL_0063, CRL-3216) were purchased from the American Type Culture Collection.. AML-12 cells were cultured in DMEM/F-12 medium (Gibco) supplemented with 10% FBS (Thermo Fisher Scientific), 5 mg ml−1 insulin, 5 μg ml−1 transferrin, 5 ng ml−1 selenium, 40 ng ml−1 dexamethasone and 1% penicillin–streptomycin (Thermo Fisher Scientific).AML-12 cells were cultured in DMEM/F-12 medium (Gibco) supplemented with 10% FBS (Thermo Fisher Scientific), 5 mg ml−1 insulin, 5 μg ml−1 transferrin, 5 ng ml−1 selenium, 40 ng ml−1 dexamethasone and 1% penicillin–streptomycin (Thermo Fisher Scientific).

    Article Title: LINC00659 Inhibits Hepatocellular Carcinoma Malignant Progression by Blocking Aerobic Glycolysis through FUS Recruitment and SLC10A1 Modulation.
    Article Snippet: Cell Culture.Cell Culture.. Human normal hepatocytes (THLE-2) were purchased from ATCC (CRL-2706, Manassas, VA, USA), and human HCC cell lines (HepG2, HuH-7, and Li7) were acquired from Procell (CL-0103; CL-0120; CL0139, Wuhan, Hubei, China).. DMEM (CM-0120, Procell, Wuhan, Hubei, China) containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin was used for HepG2, HuH-7, and Li-7 cells.DMEM (CM-0120, Procell, Wuhan, Hubei, China) containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin was used for HepG2, HuH-7, and Li-7 cells.

    Article Title: ETS-1/c-Met drives resistance to sorafenib in hepatocellular carcinoma
    Article Snippet: All tissue samples were fixed in formalin and embedded in paraffin in compliance with the declaration of Helsinki.All tissue samples were fixed in formalin and embedded in paraffin in compliance with the declaration of Helsinki.. The human HCC cell lines Huh-7, SK-Hep1, HepG2, and human normal hepatocytes THLE-2 and HHL-5 were purchased from the American Type Culture Collection (Rockville, MD, USA).. Huh-7 R (acquired sorafenib-resistant HCC cell line) was established from the Huh-7 cell line.Huh-7 R (acquired sorafenib-resistant HCC cell line) was established from the Huh-7 cell line.

    Article Title: LINC00659 Inhibits Hepatocellular Carcinoma Malignant Progression by Blocking Aerobic Glycolysis through FUS Recruitment and SLC10A1 Modulation
    Article Snippet: Human normal hepatocytes (THLE-2) were purchased from ATCC (CRL-2706, Manassas, VA, USA), and human HCC cell lines (HepG2, HuH-7, and Li-7) were acquired from Procell (CL-0103; CL-0120; CL-0139, Wuhan, Hubei, China). .. D

    Quantitative RT-PCR:

    Article Title: Construction of a Prognostic Model Based on Insulin Resistance-Related Genes to Predict TACE Response and Identification of PD-98059 as a Potential Therapeutic Agent
    Article Snippet: Human normal hepatocytes THLE-2, hepatoma cells Huh-7, HepG2, Hep 3 B, SNU-387, and SK-Hep-1 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Among them, Hep3B is p53-deficient, HepG2 is wild-type p53, and retains many hepatocyte-like metabolic functions., Simultaneously, they were derived from HBV-related HCC.Among them, Hep3B is p53-deficient, HepG2 is wild-type p53, and retains many hepatocyte-like metabolic functions., Simultaneously, they were derived from HBV-related HCC.

    Article Title: Author Correction: Intestinal TM6SF2 protects against metabolic dysfunction-associated steatohepatitis through the gut–liver axis
    Article Snippet: Mouse normal hepatocytes AML-12 cells (RRID: CVCL_0140, CRL-2254), human normal hepatocytes THLE-2 (RRID: CVCL_3803, CRL-2706), human colon epithelial cells Caco2 (RRID: CVCL_0025, HTB-37) and human HEK293T cells (RRID: CVCL_0063, CRL-3216) were purchased from the American Type Culture Collection.. AML-12 cells were cultured in DMEM/F-12 medium (Gibco) supplemented with 10% FBS (Thermo Fisher Scientific), 5 mg ml−1 insulin, 5 μg ml−1 transferrin, 5 ng ml−1 selenium, 40 ng ml−1 dexamethasone and 1% penicillin–streptomycin (Thermo Fisher Scientific).AML-12 cells were cultured in DMEM/F-12 medium (Gibco) supplemented with 10% FBS (Thermo Fisher Scientific), 5 mg ml−1 insulin, 5 μg ml−1 transferrin, 5 ng ml−1 selenium, 40 ng ml−1 dexamethasone and 1% penicillin–streptomycin (Thermo Fisher Scientific).

    Article Title: Intestinal TM6SF2 protects against metabolic dysfunction-associated steatohepatitis through the gut–liver axis
    Article Snippet: Mouse normal hepatocytes AML-12 cells (RRID: CVCL_0140, CRL-2254), human normal hepatocytes THLE-2 (RRID: CVCL_3803, CRL-2706), human colon epithelial cells Caco2 (RRID: CVCL_0025, HTB-37) and human HEK293T cells (RRID: CVCL_0063, CRL-3216) were purchased from the American Type Culture Collection.. AML-12 cells were cultured in DMEM/F-12 medium (Gibco) supplemented with 10% FBS (Thermo Fisher Scientific), 5 mg ml −1 insulin, 5 μg ml −1 transferrin, 5 ng ml −1 selenium, 40 ng ml −1 dexamethasone and 1% penicillin–streptomycin (Thermo Fisher Scientific).AML-12 cells were cultured in DMEM/F-12 medium (Gibco) supplemented with 10% FBS (Thermo Fisher Scientific), 5 mg ml −1 insulin, 5 μg ml −1 transferrin, 5 ng ml −1 selenium, 40 ng ml −1 dexamethasone and 1% penicillin–streptomycin (Thermo Fisher Scientific).

    Article Title: Intestinal TM6SF2 protects against metabolic dysfunction-associated steatohepatitis through the gut-liver axis.
    Article Snippet: Mouse normal hepatocytes AML-12 cells (RRID: CVCL_0140, CRL-2254), human normal hepatocytes THLE-2 (RRID: CVCL_3803, CRL-2706), human colon epithelial cells Caco2 (RRID: CVCL_0025, HTB-37) and human HEK293T cells (RRID: CVCL_0063, CRL-3216) were purchased from the American Type Culture Collection.. AML-12 cells were cultured in DMEM/F-12 medium (Gibco) supplemented with 10% FBS (Thermo Fisher Scientific), 5 mg ml−1 insulin, 5 μg ml−1 transferrin, 5 ng ml−1 selenium, 40 ng ml−1 dexamethasone and 1% penicillin–streptomycin (Thermo Fisher Scientific).AML-12 cells were cultured in DMEM/F-12 medium (Gibco) supplemented with 10% FBS (Thermo Fisher Scientific), 5 mg ml−1 insulin, 5 μg ml−1 transferrin, 5 ng ml−1 selenium, 40 ng ml−1 dexamethasone and 1% penicillin–streptomycin (Thermo Fisher Scientific).

    Article Title: LINC00659 Inhibits Hepatocellular Carcinoma Malignant Progression by Blocking Aerobic Glycolysis through FUS Recruitment and SLC10A1 Modulation.
    Article Snippet: Cell Culture.Cell Culture.. Human normal hepatocytes (THLE-2) were purchased from ATCC (CRL-2706, Manassas, VA, USA), and human HCC cell lines (HepG2, HuH-7, and Li7) were acquired from Procell (CL-0103; CL-0120; CL0139, Wuhan, Hubei, China).. DMEM (CM-0120, Procell, Wuhan, Hubei, China) containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin was used for HepG2, HuH-7, and Li-7 cells.DMEM (CM-0120, Procell, Wuhan, Hubei, China) containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin was used for HepG2, HuH-7, and Li-7 cells.

    Article Title: ETS-1/c-Met drives resistance to sorafenib in hepatocellular carcinoma
    Article Snippet: All tissue samples were fixed in formalin and embedded in paraffin in compliance with the declaration of Helsinki.All tissue samples were fixed in formalin and embedded in paraffin in compliance with the declaration of Helsinki.. The human HCC cell lines Huh-7, SK-Hep1, HepG2, and human normal hepatocytes THLE-2 and HHL-5 were purchased from the American Type Culture Collection (Rockville, MD, USA).. Huh-7 R (acquired sorafenib-resistant HCC cell line) was established from the Huh-7 cell line.Huh-7 R (acquired sorafenib-resistant HCC cell line) was established from the Huh-7 cell line.

    Article Title: LINC00659 Inhibits Hepatocellular Carcinoma Malignant Progression by Blocking Aerobic Glycolysis through FUS Recruitment and SLC10A1 Modulation
    Article Snippet: Human normal hepatocytes (THLE-2) were purchased from ATCC (CRL-2706, Manassas, VA, USA), and human HCC cell lines (HepG2, HuH-7, and Li-7) were acquired from Procell (CL-0103; CL-0120; CL-0139, Wuhan, Hubei, China). .. D



    Similar Products

    human normal hepatocytes thle 2  (ATCC)
    98
    ATCC human normal hepatocytes thle 2
    The selection of liver cancer cell lines and the WST-8 drug susceptibility test validated the validity of the prognostic features gene. ( A – D ) The mRNA expressions of the four molecules were detected <t>in</t> <t>THLE-2</t> and five liver cancer cell lines using qRT-PCR. ( E and F ) IC50 of lobaplatin for Hep 3B detected in normoxic and hypoxic environments using WST-8. ( G and H ) The IC50 of lobaplatin in SNU-387 cells treated with lobaplatin in normoxic and hypoxic environments was determined by WST-8 method. **P<0.01, ***P<0.001.
    Human Normal Hepatocytes Thle 2, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+normal+hepatocytes+thle+2/THLE-2/pmc13003803-84-0-18
    Average 98 stars, based on 1 article reviews
    human normal hepatocytes thle 2 - by Bioz Stars, 2026-09
    98/100 stars
    		  Buy from Supplier
    human normal hepatocyte thle 2  (ATCC)
    98
    ATCC human normal hepatocyte thle 2
    The selection of liver cancer cell lines and the WST-8 drug susceptibility test validated the validity of the prognostic features gene. ( A – D ) The mRNA expressions of the four molecules were detected <t>in</t> <t>THLE-2</t> and five liver cancer cell lines using qRT-PCR. ( E and F ) IC50 of lobaplatin for Hep 3B detected in normoxic and hypoxic environments using WST-8. ( G and H ) The IC50 of lobaplatin in SNU-387 cells treated with lobaplatin in normoxic and hypoxic environments was determined by WST-8 method. **P<0.01, ***P<0.001.
    Human Normal Hepatocyte Thle 2, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+normal+hepatocytes+thle+2/THLE-2/pm41201090-275-9-33
    Average 98 stars, based on 1 article reviews
    human normal hepatocyte thle 2 - by Bioz Stars, 2026-09
    98/100 stars
    		  Buy from Supplier
    human normal hepatocytes thle2  (ATCC)
    98
    ATCC human normal hepatocytes thle2
    UGP ameliorates palmitatic acid-induced lipotoxic hepatocyte injury. Effects of palmitatic acid stimulation and Fer-1, UGP and TGP treatment of on human normal hepatocyte <t>THLE2</t> cell viability was examined using CCK8 assay (A) and LDH assay (B) (n = 4). (C) Effects of palmitatic acid stimulation and Fer-1, UGP and TGP treatment of on THLE2 cell damage was determined by ALT assay (n = 4). (D) Cell death assay was performed using Annexin V/PI staining (n = 4). Annexin V + PI − , Annexin V + PI − , and Annexin V + PI + cells were considered dead cells. Representative fluorescent images and quantification of DCFDA (2′,7′-dichlorofluorescein diacetate) (E) and Mito-Tracker Red CMXRos (F) staining for ROS and mitochondrial membrane potential detection (scale bar = 100 μm, n = 4). (G) Flow cytometry analysis of lipid peroxidation by Liperfluo staining on THLE2 cells. (H) Representative immunofluorescent images of ACTA2 +ve and COL1A1 +ve cells (scale bar = 100 μm). Data are shown as box-and whisker with median (middle line), 25th–75th percentiles (box), and min-max values (whiskers), one-way ANOVA with Tukey’s correction. (ALT: Alanine transaminase; BSA:Bovine serum albumin; Fer-1: Ferrostatin-1; PAL: Palmitic acid; ROS: Reactive oxygen species; TGP: Traditional garlic powder; UGP:Ultrafine garlic powder).
    Human Normal Hepatocytes Thle2, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+normal+hepatocytes+thle+2/THLE-2/pmc12673841-96-0-14
    Average 98 stars, based on 1 article reviews
    human normal hepatocytes thle2 - by Bioz Stars, 2026-09
    98/100 stars
    		  Buy from Supplier
    human normal hepatocytes thle 2  (Procell Inc)
    86
    Procell Inc human normal hepatocytes thle 2
    UGP ameliorates palmitatic acid-induced lipotoxic hepatocyte injury. Effects of palmitatic acid stimulation and Fer-1, UGP and TGP treatment of on human normal hepatocyte <t>THLE2</t> cell viability was examined using CCK8 assay (A) and LDH assay (B) (n = 4). (C) Effects of palmitatic acid stimulation and Fer-1, UGP and TGP treatment of on THLE2 cell damage was determined by ALT assay (n = 4). (D) Cell death assay was performed using Annexin V/PI staining (n = 4). Annexin V + PI − , Annexin V + PI − , and Annexin V + PI + cells were considered dead cells. Representative fluorescent images and quantification of DCFDA (2′,7′-dichlorofluorescein diacetate) (E) and Mito-Tracker Red CMXRos (F) staining for ROS and mitochondrial membrane potential detection (scale bar = 100 μm, n = 4). (G) Flow cytometry analysis of lipid peroxidation by Liperfluo staining on THLE2 cells. (H) Representative immunofluorescent images of ACTA2 +ve and COL1A1 +ve cells (scale bar = 100 μm). Data are shown as box-and whisker with median (middle line), 25th–75th percentiles (box), and min-max values (whiskers), one-way ANOVA with Tukey’s correction. (ALT: Alanine transaminase; BSA:Bovine serum albumin; Fer-1: Ferrostatin-1; PAL: Palmitic acid; ROS: Reactive oxygen species; TGP: Traditional garlic powder; UGP:Ultrafine garlic powder).
    Human Normal Hepatocytes Thle 2, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+normal+hepatocytes+thle+2/2+thle/pm41207513-56-7-14
    Average 86 stars, based on 1 article reviews
    human normal hepatocytes thle 2 - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    human normal hepatocyte thle 2 cells  (Procell Inc)
    86
    Procell Inc human normal hepatocyte thle 2 cells
    UGP ameliorates palmitatic acid-induced lipotoxic hepatocyte injury. Effects of palmitatic acid stimulation and Fer-1, UGP and TGP treatment of on human normal hepatocyte <t>THLE2</t> cell viability was examined using CCK8 assay (A) and LDH assay (B) (n = 4). (C) Effects of palmitatic acid stimulation and Fer-1, UGP and TGP treatment of on THLE2 cell damage was determined by ALT assay (n = 4). (D) Cell death assay was performed using Annexin V/PI staining (n = 4). Annexin V + PI − , Annexin V + PI − , and Annexin V + PI + cells were considered dead cells. Representative fluorescent images and quantification of DCFDA (2′,7′-dichlorofluorescein diacetate) (E) and Mito-Tracker Red CMXRos (F) staining for ROS and mitochondrial membrane potential detection (scale bar = 100 μm, n = 4). (G) Flow cytometry analysis of lipid peroxidation by Liperfluo staining on THLE2 cells. (H) Representative immunofluorescent images of ACTA2 +ve and COL1A1 +ve cells (scale bar = 100 μm). Data are shown as box-and whisker with median (middle line), 25th–75th percentiles (box), and min-max values (whiskers), one-way ANOVA with Tukey’s correction. (ALT: Alanine transaminase; BSA:Bovine serum albumin; Fer-1: Ferrostatin-1; PAL: Palmitic acid; ROS: Reactive oxygen species; TGP: Traditional garlic powder; UGP:Ultrafine garlic powder).
    Human Normal Hepatocyte Thle 2 Cells, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+normal+hepatocytes+thle+2/2+thle/pmc12392558-29-0-16
    Average 86 stars, based on 1 article reviews
    human normal hepatocyte thle 2 cells - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    normal human hepatocytes  (ATCC)
    98
    ATCC normal human hepatocytes
    UGP ameliorates palmitatic acid-induced lipotoxic hepatocyte injury. Effects of palmitatic acid stimulation and Fer-1, UGP and TGP treatment of on human normal hepatocyte <t>THLE2</t> cell viability was examined using CCK8 assay (A) and LDH assay (B) (n = 4). (C) Effects of palmitatic acid stimulation and Fer-1, UGP and TGP treatment of on THLE2 cell damage was determined by ALT assay (n = 4). (D) Cell death assay was performed using Annexin V/PI staining (n = 4). Annexin V + PI − , Annexin V + PI − , and Annexin V + PI + cells were considered dead cells. Representative fluorescent images and quantification of DCFDA (2′,7′-dichlorofluorescein diacetate) (E) and Mito-Tracker Red CMXRos (F) staining for ROS and mitochondrial membrane potential detection (scale bar = 100 μm, n = 4). (G) Flow cytometry analysis of lipid peroxidation by Liperfluo staining on THLE2 cells. (H) Representative immunofluorescent images of ACTA2 +ve and COL1A1 +ve cells (scale bar = 100 μm). Data are shown as box-and whisker with median (middle line), 25th–75th percentiles (box), and min-max values (whiskers), one-way ANOVA with Tukey’s correction. (ALT: Alanine transaminase; BSA:Bovine serum albumin; Fer-1: Ferrostatin-1; PAL: Palmitic acid; ROS: Reactive oxygen species; TGP: Traditional garlic powder; UGP:Ultrafine garlic powder).
    Normal Human Hepatocytes, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+normal+hepatocytes+thle+2/THLE-2/pm39968051-69-0-3
    Average 98 stars, based on 1 article reviews
    normal human hepatocytes - by Bioz Stars, 2026-09
    98/100 stars
    		  Buy from Supplier

    Image Search Results


    The selection of liver cancer cell lines and the WST-8 drug susceptibility test validated the validity of the prognostic features gene. ( A – D ) The mRNA expressions of the four molecules were detected in THLE-2 and five liver cancer cell lines using qRT-PCR. ( E and F ) IC50 of lobaplatin for Hep 3B detected in normoxic and hypoxic environments using WST-8. ( G and H ) The IC50 of lobaplatin in SNU-387 cells treated with lobaplatin in normoxic and hypoxic environments was determined by WST-8 method. **P<0.01, ***P<0.001.

    Journal: Journal of Hepatocellular Carcinoma

    Article Title: Construction of a Prognostic Model Based on Insulin Resistance-Related Genes to Predict TACE Response and Identification of PD-98059 as a Potential Therapeutic Agent

    doi: 10.2147/JHC.S553710

    Figure Lengend Snippet: The selection of liver cancer cell lines and the WST-8 drug susceptibility test validated the validity of the prognostic features gene. ( A – D ) The mRNA expressions of the four molecules were detected in THLE-2 and five liver cancer cell lines using qRT-PCR. ( E and F ) IC50 of lobaplatin for Hep 3B detected in normoxic and hypoxic environments using WST-8. ( G and H ) The IC50 of lobaplatin in SNU-387 cells treated with lobaplatin in normoxic and hypoxic environments was determined by WST-8 method. **P<0.01, ***P<0.001.

    Article Snippet: Human normal hepatocytes THLE-2, hepatoma cells Huh-7, HepG2, Hep 3 B, SNU-387, and SK-Hep-1 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Selection, Quantitative RT-PCR

    UGP ameliorates palmitatic acid-induced lipotoxic hepatocyte injury. Effects of palmitatic acid stimulation and Fer-1, UGP and TGP treatment of on human normal hepatocyte THLE2 cell viability was examined using CCK8 assay (A) and LDH assay (B) (n = 4). (C) Effects of palmitatic acid stimulation and Fer-1, UGP and TGP treatment of on THLE2 cell damage was determined by ALT assay (n = 4). (D) Cell death assay was performed using Annexin V/PI staining (n = 4). Annexin V + PI − , Annexin V + PI − , and Annexin V + PI + cells were considered dead cells. Representative fluorescent images and quantification of DCFDA (2′,7′-dichlorofluorescein diacetate) (E) and Mito-Tracker Red CMXRos (F) staining for ROS and mitochondrial membrane potential detection (scale bar = 100 μm, n = 4). (G) Flow cytometry analysis of lipid peroxidation by Liperfluo staining on THLE2 cells. (H) Representative immunofluorescent images of ACTA2 +ve and COL1A1 +ve cells (scale bar = 100 μm). Data are shown as box-and whisker with median (middle line), 25th–75th percentiles (box), and min-max values (whiskers), one-way ANOVA with Tukey’s correction. (ALT: Alanine transaminase; BSA:Bovine serum albumin; Fer-1: Ferrostatin-1; PAL: Palmitic acid; ROS: Reactive oxygen species; TGP: Traditional garlic powder; UGP:Ultrafine garlic powder).

    Journal: Frontiers in Pharmacology

    Article Title: Ultrafine garlic powder alleviates non-alcoholic steatohepatitis by inhibiting hepatocyte ferroptosis and modulating ERK-dependent oxidative stress

    doi: 10.3389/fphar.2025.1711917

    Figure Lengend Snippet: UGP ameliorates palmitatic acid-induced lipotoxic hepatocyte injury. Effects of palmitatic acid stimulation and Fer-1, UGP and TGP treatment of on human normal hepatocyte THLE2 cell viability was examined using CCK8 assay (A) and LDH assay (B) (n = 4). (C) Effects of palmitatic acid stimulation and Fer-1, UGP and TGP treatment of on THLE2 cell damage was determined by ALT assay (n = 4). (D) Cell death assay was performed using Annexin V/PI staining (n = 4). Annexin V + PI − , Annexin V + PI − , and Annexin V + PI + cells were considered dead cells. Representative fluorescent images and quantification of DCFDA (2′,7′-dichlorofluorescein diacetate) (E) and Mito-Tracker Red CMXRos (F) staining for ROS and mitochondrial membrane potential detection (scale bar = 100 μm, n = 4). (G) Flow cytometry analysis of lipid peroxidation by Liperfluo staining on THLE2 cells. (H) Representative immunofluorescent images of ACTA2 +ve and COL1A1 +ve cells (scale bar = 100 μm). Data are shown as box-and whisker with median (middle line), 25th–75th percentiles (box), and min-max values (whiskers), one-way ANOVA with Tukey’s correction. (ALT: Alanine transaminase; BSA:Bovine serum albumin; Fer-1: Ferrostatin-1; PAL: Palmitic acid; ROS: Reactive oxygen species; TGP: Traditional garlic powder; UGP:Ultrafine garlic powder).

    Article Snippet: Human normal hepatocytes (THLE2) and hepatic stellate cells (HSCs) (LX-2) were purchased from the American Type Culture Collection (ATCC) and cultured in BEGM kit medium and RPMI medium supplemented with 10% FBS, respectively.

    Techniques: CCK-8 Assay, Lactate Dehydrogenase Assay, Staining, Membrane, Flow Cytometry, Whisker Assay

    UGP significantly ameliorates erastin-induced hepatocyte ferroptosis. Effects of erastin stimulation and Fer-1, UGP and TGP treatment of on THLE2 cell viability was examined using CCK8 assay (A) and LDH assay (B) (n = 4). (C) Effects of erastin stimulation and Fer-1, UGP and TGP treatment of on THLE2 cell damage was determined by ALT assay (n = 4). (D) Cell death assay was performed using Annexin V/PI staining (n = 4). Annexin V + PI − , Annexin V + PI − , and Annexin V + PI + cells were considered dead cells. (E) Flow cytometry analysis of cellular Fe 2+ levels by FerroOrange staining on THLE2 cells. Representative fluorescent images and quantification of DCFDA (2′,7′-dichlorofluorescein diacetate) (F) and Mito-Tracker Red CMXRos (G) staining for ROS and mitochondrial membrane potential detection (scale bar = 100 μm, n = 4). (H) Flow cytometry analysis of lipid peroxidation by Liperfluo staining on THLE2 cells. Data are shown as box-and whisker with median (middle line), 25th-75th percentiles (box), and min-max values (whiskers), one-way ANOVA with Tukey’s correction. (ALT: Alanine transaminase; ERA: Erastin; Fer-1: Ferrostatin-1; ROS: Reactive oxygen species; TGP: Traditional garlic powder; UGP: Ultrafine garlic powder).

    Journal: Frontiers in Pharmacology

    Article Title: Ultrafine garlic powder alleviates non-alcoholic steatohepatitis by inhibiting hepatocyte ferroptosis and modulating ERK-dependent oxidative stress

    doi: 10.3389/fphar.2025.1711917

    Figure Lengend Snippet: UGP significantly ameliorates erastin-induced hepatocyte ferroptosis. Effects of erastin stimulation and Fer-1, UGP and TGP treatment of on THLE2 cell viability was examined using CCK8 assay (A) and LDH assay (B) (n = 4). (C) Effects of erastin stimulation and Fer-1, UGP and TGP treatment of on THLE2 cell damage was determined by ALT assay (n = 4). (D) Cell death assay was performed using Annexin V/PI staining (n = 4). Annexin V + PI − , Annexin V + PI − , and Annexin V + PI + cells were considered dead cells. (E) Flow cytometry analysis of cellular Fe 2+ levels by FerroOrange staining on THLE2 cells. Representative fluorescent images and quantification of DCFDA (2′,7′-dichlorofluorescein diacetate) (F) and Mito-Tracker Red CMXRos (G) staining for ROS and mitochondrial membrane potential detection (scale bar = 100 μm, n = 4). (H) Flow cytometry analysis of lipid peroxidation by Liperfluo staining on THLE2 cells. Data are shown as box-and whisker with median (middle line), 25th-75th percentiles (box), and min-max values (whiskers), one-way ANOVA with Tukey’s correction. (ALT: Alanine transaminase; ERA: Erastin; Fer-1: Ferrostatin-1; ROS: Reactive oxygen species; TGP: Traditional garlic powder; UGP: Ultrafine garlic powder).

    Article Snippet: Human normal hepatocytes (THLE2) and hepatic stellate cells (HSCs) (LX-2) were purchased from the American Type Culture Collection (ATCC) and cultured in BEGM kit medium and RPMI medium supplemented with 10% FBS, respectively.

    Techniques: CCK-8 Assay, Lactate Dehydrogenase Assay, Staining, Flow Cytometry, Membrane, Whisker Assay